Page Header

Determination of heavy metals in soil by inductively coupled plasma mass spectrometry (ICP-MS) with internal standard method

Jingchun Mao, Xinyue Liu, Bao Chen, Famei Luo, Xudong Wu, Donghua Jiang, Zhenggang Luo


Soil ,the carrier of agricultural production and important part of the ecological environment, is heavily contaminated with hazards heavy metals. Therefore, it is oblige to research analytical techniques that could efficiently determine the total content of heavy metals in soil. The determination of heavy metals in soil was disturbed by matrix elements or spectral interferences . In this study , this problem was solved by internal standard method . GBW07402、GBW07448、GBW07423、GBW07428、GBW074079 soil sample were chosen to be the Certified Reference Materials, soils was prepared by microwave digestion with mixed acid following analyzed for determination the content(Cr,Cu, Pb,Ba,Ni,Mn ) by Inductively coupled plasma mass spectrometric in 50ug/L internal standard concentration, the method was validated by compared with certified values 、method contrast(standard addition method versus internal standard method scan the same prepared solution ) and recovery check. The results of internal standard method are in excellent agreement with the indicative values and the date obtained from standard addition method, respectively. Recoveries were adequate being in the acceptable range of 90-99% and RSD of <6.7 % for all the elements at three level of 5,20 and 50mg/kg with quantified by standard addition method and internal standard method .Finally, The graphy of quality control(n=100)were obtained to guide internal quality control in laboratory


Soil; Heavy metal ; inductively coupled plasma mass spectrometry; internal standard method

Full Text:



NWZ Najib,SA Mohammed,SH Ismail. Assessment of Heavy Metal in Soil due to Human Activities in Kangar, Perlis, Malaysia. International Journal of Civil & Environmental, 2012, 12(6):28-33.

V Kumar , AK Chopra. Distribution, Enrichment and Accumulation of Heavy Metals in Soil and Trigonella foenum-graecum L. (Fenugreek) after Fertigation with Paper Mill Effluent.Open Journal of Metal , 2013, 3(2A):8-20

Gennaro Brunetti , Karam Farrag, Pedro SolerRovira. Heavy metals accumulation and distribution in durum wheat and barley grown in contaminated soils under Mediterranean field conditions. Journal of Plant Interactions, 2012 , 7(2):160-174

Y Cui,YS Ding,WM Gong. Study on the correlation between the chemical forms of the heavy metals in soil and the metal uptake by plant. Journal of Dalian Maritime University , 2005 , 31(2):59-63

R Clemente , DJ Walker,A Roig. Heavy metal bioavailability in a soil affected by mineral sulphides contamination following the mine spillage at Aznalcóllar (Spain). Biodegradation , 2003, 14(3):199-205

H Zhao, B Xia,C Fan. Human health risk from soil heavy metal contamination under different land uses near Dabaoshan Mine, Southern China. Science of the Total Environment , 2012, 417-418(7385):45.

P Zhuang, MB Mcbride,H Xia. Health risk from heavy metals via consumption of food crops in the vicinity of Dabaoshan mine, South China. Science of the Total Environment , 2009 , 407 (5):1551-1561

YYi, Z Yang,S Zhang . Ecological risk assessment of heavy metals in sediment and humanhealth risk assessment of heavy metals in fishes in the middle and lower reaches of theYangtze River basin . Environmental Pollution, 2011, 159 (10) :2575-2585

S Muhammad, MT Shah,S Khan.Health risk assessment of heavy metals and their source apportionment in drinking water of Kohistan region, northern Pakistan. Microchemical Journal, 2011 , 98 (2) :334-343

Peijun Li , XinWang, Graeme Allinson. Risk assessment of heavy metals in soil previously irrigated with industrial wastewater in Shenyang. Journal of Hazardous Materials 161 (2009) 516–521

Rajneet Kour Soodan, Yogesh B.Pakade,Avinash Nagpal.Analytical techniquesfor estimation of heavy metals in soil ecosystem: A tabulated review. Talanta, 2014,125 (11) :405.

ŞERIE TOKALIOLU, ŞENOL KARTAL,and ALFER A. GfhJEŞ. Determination of Heavy Metals in SoilExtracts and Plant Tissues at Around of a Zinc Smelter. International Journal ofEnvironmental Analytical Chemistry,2006, 80(3):201-217.

Sylvia Gwebu, Nikita T. Tavengwa, Michael J. Klink. Quantification of Cd, Cu, Pb and Zn from sewage sludge by modified-BCR and ultrasound assisted-modified BCR sequential extraction methods. African Journal of Pure and Applied Chemistry, 11(2), 2017:9-18.

Zhaoyong Zhang, Jilili·Abuduwaili,Fengqing Jiang.Determination of occurrence characteristics of heavy metals in soil and water environments in Tianshan Mountains, central Asia. Analytical Letters, 2013 , 46 (13) :2122-2131.

Samir Ghannem, Abdelhafidh Khazri, Badreddine Sellami. Assessment of heavy metal contamination in soil and Chlaenius (Chlaeniellus) olivieri (Coleoptera, Carabidae) in the vicinity of a textile factory near Ras Jbel (Bizerte, Tunisia). Environmental Earth Sciences, 2016, 75:442..

Jan Goossens, Luc Moens, Richard Dams . Inductively coupled plasma mass spectrometric determination of heavy metals in soil and sludge candidate reference materials. Analytica Chimica Acta ,1995(304) :307-315.

X Wang,Y Zhang,N Liu. Determination of heavy metal ions in soil by ICP-MS with microwave digestion. Chinese Journal of Spectroscopy Laboratory, 2008, 25(6):1183-1187.

EHS Bo,BR Kowalski. Generalized standard addition method. Analytical Chemistry, 1979 , 51 (7):1031-1038.

ET Tipper, P Louvat, F Capmas. Accuracy of stable Mg and Ca isotope data obtained by MC-ICP-MS using the standard addition method. Chemical Geology, 2008, 257(1–2):65-75

Certification of Geochemical reference materials .1982-2012.

M Thompson , R Wood. Harmonized guidelines for internal quality control in analytical chemistry laboratories (Technical Report).Pure & Applied Chemistry, 1995, 67(4):649-666.

JO Westgard, T Groth, T Aronsson. Performance characteristics of rules for internal quality control: probabilities for false rejection and error detection. Clinical Chemistry, 1977, 23 (10):1857-67



  • There are currently no refbacks.

Copyright (c) 2017 Jingchun Mao

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.